Review




Structured Review

GeneTex rabbit anti-eif4e2
Rabbit Anti Eif4e2, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/anti+eif4e2/bio_rxiv__2025__02__28__640929-77-31-34
Average 90 stars, based on 1 article reviews
rabbit anti-eif4e2 - by Bioz Stars, 2026-08
90/100 stars

Images



Similar Products

90
GeneTex rabbit anti-eif4e2
Rabbit Anti Eif4e2, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/anti+eif4e2/bio_rxiv__2025__02__28__640929-77-31-34
Average 90 stars, based on 1 article reviews
rabbit anti-eif4e2 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

90
GeneTex rabbit anti- eif4e2
Rabbit Anti Eif4e2, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/anti+eif4e2/pm39018414-291-17-20
Average 90 stars, based on 1 article reviews
rabbit anti- eif4e2 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

93
Proteintech rabbit anti eif4e2 4ehp
Rabbit Anti Eif4e2 4ehp, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/EIF4E2+Antibody/bio_rxiv__2023__11__20__565808-218-7-10
Average 93 stars, based on 1 article reviews
rabbit anti eif4e2 4ehp - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier

94
Cell Signaling Technology Inc anti eif4e2
Anti Eif4e2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/4EHP+Rabbit+mAb/pmc10528759-24-0-2
Average 94 stars, based on 1 article reviews
anti eif4e2 - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier

94
Cell Signaling Technology Inc rabbit monoclonal anti 4ehp eif4e2 cell signaling technology
Fig. 1 Expression of Nsp2 and its binding to <t>eIF4E2.</t> A Representative image of western blot analysis of expression of Strep-Nsp2 in HEK293T cells stably transfected with either Lipofectamine-3000 or LTX. B Biotin-Agarose pull-down. Left, western blot analyses of eIF4E2 (upper panel) and Nsp2 (lower panel). Right, graph represents densitometric ratio of eIF4E2 to Nsp2. C m7GTP-Sepharose pull-down. Left, western blot analyses of Nsp2 (upper panel) and eIF4E2 (lower). Right, graph represents densitometric ratio of Nsp2 to eIF4E2. C—control; N—normoxia; H—hypoxia. The blots shown are representative of three completely independent experiments
Rabbit Monoclonal Anti 4ehp Eif4e2 Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/4EHP+Rabbit+mAb/pm36998012-310-0-4
Average 94 stars, based on 1 article reviews
rabbit monoclonal anti 4ehp eif4e2 cell signaling technology - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier

94
Cell Signaling Technology Inc rabbit monoclonal anti 4ehp eif4e2
Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, <t>eIF4E2,</t> and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal ( A ) and hypoxic ( B ) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal
Rabbit Monoclonal Anti 4ehp Eif4e2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/4EHP+Rabbit+mAb/pmc10060939-1-0-5
Average 94 stars, based on 1 article reviews
rabbit monoclonal anti 4ehp eif4e2 - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier

90
Thermo Fisher rabbit anti-eif4e2
Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, <t>eIF4E2,</t> and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal ( A ) and hypoxic ( B ) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal
Rabbit Anti Eif4e2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/pmc10060939-5-0-3
Average 90 stars, based on 1 article reviews
rabbit anti-eif4e2 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

90
GeneTex rabbit anti-eif4e2 gtx103977
Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, <t>eIF4E2,</t> and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal ( A ) and hypoxic ( B ) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal
Rabbit Anti Eif4e2 Gtx103977, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti-eif4e2/anti+eif4e2/pmc09371684__pnas__2204539119__sapp-5-26-28
Average 90 stars, based on 1 article reviews
rabbit anti-eif4e2 gtx103977 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


Fig. 1 Expression of Nsp2 and its binding to eIF4E2. A Representative image of western blot analysis of expression of Strep-Nsp2 in HEK293T cells stably transfected with either Lipofectamine-3000 or LTX. B Biotin-Agarose pull-down. Left, western blot analyses of eIF4E2 (upper panel) and Nsp2 (lower panel). Right, graph represents densitometric ratio of eIF4E2 to Nsp2. C m7GTP-Sepharose pull-down. Left, western blot analyses of Nsp2 (upper panel) and eIF4E2 (lower). Right, graph represents densitometric ratio of Nsp2 to eIF4E2. C—control; N—normoxia; H—hypoxia. The blots shown are representative of three completely independent experiments

Journal: Virology journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions.

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Fig. 1 Expression of Nsp2 and its binding to eIF4E2. A Representative image of western blot analysis of expression of Strep-Nsp2 in HEK293T cells stably transfected with either Lipofectamine-3000 or LTX. B Biotin-Agarose pull-down. Left, western blot analyses of eIF4E2 (upper panel) and Nsp2 (lower panel). Right, graph represents densitometric ratio of eIF4E2 to Nsp2. C m7GTP-Sepharose pull-down. Left, western blot analyses of Nsp2 (upper panel) and eIF4E2 (lower). Right, graph represents densitometric ratio of Nsp2 to eIF4E2. C—control; N—normoxia; H—hypoxia. The blots shown are representative of three completely independent experiments

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) Cell signaling Technology Cat# 6916, RRID: AB_10839268 Mouse monoclonal anti-FGF2 Santa Cruz Biotechnology Cat# 145 Rabbit monoclonal anti-GAPDH Cell signaling Technology Cat# 2118, RRID: AB_561053 Mouse anti-strep Invitrogen Cat# MA5-17,283 Rabbit anti-eIF4E2 Invitrogen Cat# PA5-110849 Rabbit Calnexin Invitrogen Cat# PA5-34754 Rabbit RbS3 Cell signal tech Cat# 2579S Goat anti-Mouse Alexa FluorTM 488 Life Technologies Cat# A11029 Goat anti-Rabbit Alexa FluorTM 594 Life Technologies Cat# A11012 Rabbit polyclonal antiP-mTOR (S2448) Cell signaling Technology Cat# 2971,RRID: AB__330970 Rabbit monoclonal anti-mTOR Cell signaling Technology Cat# 2983,RRID: AB_ 2105622 Rabbit polyclonal antiP-4EBP(S65) Cell signaling Technology Cat# 9451,RRID: AB_330947 Rabbit polyclonal anti4EBP (PHAS-1) Invitrogen Cat# 51-2900 Rabbit monoclonal anti-P-eIF2α (Ser51) Cell signaling Technology Cat#9721,RRID: AB_330951 rabbit monoclonal anti-eIF2α(D7D3) XP Cell signaling Technology Cat#9721,RRID: AB_10692650 Mouse monoclonal anti-eIF4E2 (Figs. 6, 7, and Additional file 1: Figure S1) Invitrogen Cat#MA5-25412 Mouse monoclonal anti-GAPDH (Fig. 5C and Additional file 1: Figure S1) Fitzgerald Cat# 10R-G109A, RRID: AB 1285808 Goat polyclonal antiVEGF-C R & D systems Cat# AF752-SP Rabbit polyclonal antiRibosomal protein L 7a (E109) Cell signaling Technology Cat# 2415,RRID:AB_ 2182059 Rabbit polyclonal anti-eIF4G Gift from Dr. Rhoads (LSUHSC, Shreveport) Mouse monoclonal anti-eIF4A Rabbit polyclonal anti-Actin Sigma Cat# A2066, RRID: AB_476693 Chemicals Dulbecco’s modified eagle medium (DMEM) Sigma-Aldrich D6429 Antibiotic–Antimycotic (100X) ThermoFisher Scientific 15240-062

Techniques: Expressing, Binding Assay, Western Blot, Stable Transfection, Transfection, Control

Fig. 2 Nsp2 co-localizes with eIF4E2 and RER under normal and hypoxic conditions. A Strep-Nsp2 was probed using anti-strep antibody (green) and eIF4E2 probed using anti-eIF4E2 antibody (red), DAPI (blue) stained nuclei. Merge channel from max intensity projections of z-stacks under hypoxia shows increased co-localization (yellow). B Strep-Nsp2 (green) and calnexin (red) probed. Under hypoxia, granular staining in merged channel (yellow) shows increased co-localization from best focal plane. C Representative image of Ribosomal marker (RbS3) probed using anti-RbS3 antibody (red) and Strep-Nsp2 (green). Colocalization shown in merged channel. DAPI stained nuclei

Journal: Virology journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions.

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Fig. 2 Nsp2 co-localizes with eIF4E2 and RER under normal and hypoxic conditions. A Strep-Nsp2 was probed using anti-strep antibody (green) and eIF4E2 probed using anti-eIF4E2 antibody (red), DAPI (blue) stained nuclei. Merge channel from max intensity projections of z-stacks under hypoxia shows increased co-localization (yellow). B Strep-Nsp2 (green) and calnexin (red) probed. Under hypoxia, granular staining in merged channel (yellow) shows increased co-localization from best focal plane. C Representative image of Ribosomal marker (RbS3) probed using anti-RbS3 antibody (red) and Strep-Nsp2 (green). Colocalization shown in merged channel. DAPI stained nuclei

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) Cell signaling Technology Cat# 6916, RRID: AB_10839268 Mouse monoclonal anti-FGF2 Santa Cruz Biotechnology Cat# 145 Rabbit monoclonal anti-GAPDH Cell signaling Technology Cat# 2118, RRID: AB_561053 Mouse anti-strep Invitrogen Cat# MA5-17,283 Rabbit anti-eIF4E2 Invitrogen Cat# PA5-110849 Rabbit Calnexin Invitrogen Cat# PA5-34754 Rabbit RbS3 Cell signal tech Cat# 2579S Goat anti-Mouse Alexa FluorTM 488 Life Technologies Cat# A11029 Goat anti-Rabbit Alexa FluorTM 594 Life Technologies Cat# A11012 Rabbit polyclonal antiP-mTOR (S2448) Cell signaling Technology Cat# 2971,RRID: AB__330970 Rabbit monoclonal anti-mTOR Cell signaling Technology Cat# 2983,RRID: AB_ 2105622 Rabbit polyclonal antiP-4EBP(S65) Cell signaling Technology Cat# 9451,RRID: AB_330947 Rabbit polyclonal anti4EBP (PHAS-1) Invitrogen Cat# 51-2900 Rabbit monoclonal anti-P-eIF2α (Ser51) Cell signaling Technology Cat#9721,RRID: AB_330951 rabbit monoclonal anti-eIF2α(D7D3) XP Cell signaling Technology Cat#9721,RRID: AB_10692650 Mouse monoclonal anti-eIF4E2 (Figs. 6, 7, and Additional file 1: Figure S1) Invitrogen Cat#MA5-25412 Mouse monoclonal anti-GAPDH (Fig. 5C and Additional file 1: Figure S1) Fitzgerald Cat# 10R-G109A, RRID: AB 1285808 Goat polyclonal antiVEGF-C R & D systems Cat# AF752-SP Rabbit polyclonal antiRibosomal protein L 7a (E109) Cell signaling Technology Cat# 2415,RRID:AB_ 2182059 Rabbit polyclonal anti-eIF4G Gift from Dr. Rhoads (LSUHSC, Shreveport) Mouse monoclonal anti-eIF4A Rabbit polyclonal anti-Actin Sigma Cat# A2066, RRID: AB_476693 Chemicals Dulbecco’s modified eagle medium (DMEM) Sigma-Aldrich D6429 Antibiotic–Antimycotic (100X) ThermoFisher Scientific 15240-062

Techniques: Staining, Marker

Fig. 6 Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. 4A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, eIF4E2, and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal (A) and hypoxic (B) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal

Journal: Virology journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions.

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Fig. 6 Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. 4A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, eIF4E2, and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal (A) and hypoxic (B) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) Cell signaling Technology Cat# 6916, RRID: AB_10839268 Mouse monoclonal anti-FGF2 Santa Cruz Biotechnology Cat# 145 Rabbit monoclonal anti-GAPDH Cell signaling Technology Cat# 2118, RRID: AB_561053 Mouse anti-strep Invitrogen Cat# MA5-17,283 Rabbit anti-eIF4E2 Invitrogen Cat# PA5-110849 Rabbit Calnexin Invitrogen Cat# PA5-34754 Rabbit RbS3 Cell signal tech Cat# 2579S Goat anti-Mouse Alexa FluorTM 488 Life Technologies Cat# A11029 Goat anti-Rabbit Alexa FluorTM 594 Life Technologies Cat# A11012 Rabbit polyclonal antiP-mTOR (S2448) Cell signaling Technology Cat# 2971,RRID: AB__330970 Rabbit monoclonal anti-mTOR Cell signaling Technology Cat# 2983,RRID: AB_ 2105622 Rabbit polyclonal antiP-4EBP(S65) Cell signaling Technology Cat# 9451,RRID: AB_330947 Rabbit polyclonal anti4EBP (PHAS-1) Invitrogen Cat# 51-2900 Rabbit monoclonal anti-P-eIF2α (Ser51) Cell signaling Technology Cat#9721,RRID: AB_330951 rabbit monoclonal anti-eIF2α(D7D3) XP Cell signaling Technology Cat#9721,RRID: AB_10692650 Mouse monoclonal anti-eIF4E2 (Figs. 6, 7, and Additional file 1: Figure S1) Invitrogen Cat#MA5-25412 Mouse monoclonal anti-GAPDH (Fig. 5C and Additional file 1: Figure S1) Fitzgerald Cat# 10R-G109A, RRID: AB 1285808 Goat polyclonal antiVEGF-C R & D systems Cat# AF752-SP Rabbit polyclonal antiRibosomal protein L 7a (E109) Cell signaling Technology Cat# 2415,RRID:AB_ 2182059 Rabbit polyclonal anti-eIF4G Gift from Dr. Rhoads (LSUHSC, Shreveport) Mouse monoclonal anti-eIF4A Rabbit polyclonal anti-Actin Sigma Cat# A2066, RRID: AB_476693 Chemicals Dulbecco’s modified eagle medium (DMEM) Sigma-Aldrich D6429 Antibiotic–Antimycotic (100X) ThermoFisher Scientific 15240-062

Techniques: Western Blot, Control

Fig. 7 Nsp2 may affect composition of the cap-binding complexes. (A) Left, the representative images of western-blot analyses of proteins expressed in control and Nsp2-cells under normal and hypoxic conditions. Arrow indicates position of Strep-Nsp2. Right, ratio of densitometric signal of P-4EBP to total 4EBP in cells grown under normal or hypoxic conditions normalized to that in control cells grown under normal conditions, set as one (± SD, n = 3). (B) Left, the representative images of western blot analysis of proteins bound to m7GTP-Sepharose under normal and hypoxic conditions in control and Nsp2-cells. Arrow indicates position of Strep-Nsp2. Right, ratios of densitometric signals of eIF4G (upper graph) and eIF4E2 (lower graph) to eIF4E bound to m7GTP-Sepharose in experiments presented in lanes 1–4 (± SD, n = 3). Graph eIF4E/eIF4G: p = 0.014 for C–H versus C–N; p = 0.033 for Nsp2-H versus C–H. Graph eIF4E2/eIF4E: p = 0.03 for C–H versus C–N; p = 0.025 and 0.023 for Nsp2–H versus C–N and versus C–H, respectively

Journal: Virology journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions.

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Fig. 7 Nsp2 may affect composition of the cap-binding complexes. (A) Left, the representative images of western-blot analyses of proteins expressed in control and Nsp2-cells under normal and hypoxic conditions. Arrow indicates position of Strep-Nsp2. Right, ratio of densitometric signal of P-4EBP to total 4EBP in cells grown under normal or hypoxic conditions normalized to that in control cells grown under normal conditions, set as one (± SD, n = 3). (B) Left, the representative images of western blot analysis of proteins bound to m7GTP-Sepharose under normal and hypoxic conditions in control and Nsp2-cells. Arrow indicates position of Strep-Nsp2. Right, ratios of densitometric signals of eIF4G (upper graph) and eIF4E2 (lower graph) to eIF4E bound to m7GTP-Sepharose in experiments presented in lanes 1–4 (± SD, n = 3). Graph eIF4E/eIF4G: p = 0.014 for C–H versus C–N; p = 0.033 for Nsp2-H versus C–H. Graph eIF4E2/eIF4E: p = 0.03 for C–H versus C–N; p = 0.025 and 0.023 for Nsp2–H versus C–N and versus C–H, respectively

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) Cell signaling Technology Cat# 6916, RRID: AB_10839268 Mouse monoclonal anti-FGF2 Santa Cruz Biotechnology Cat# 145 Rabbit monoclonal anti-GAPDH Cell signaling Technology Cat# 2118, RRID: AB_561053 Mouse anti-strep Invitrogen Cat# MA5-17,283 Rabbit anti-eIF4E2 Invitrogen Cat# PA5-110849 Rabbit Calnexin Invitrogen Cat# PA5-34754 Rabbit RbS3 Cell signal tech Cat# 2579S Goat anti-Mouse Alexa FluorTM 488 Life Technologies Cat# A11029 Goat anti-Rabbit Alexa FluorTM 594 Life Technologies Cat# A11012 Rabbit polyclonal antiP-mTOR (S2448) Cell signaling Technology Cat# 2971,RRID: AB__330970 Rabbit monoclonal anti-mTOR Cell signaling Technology Cat# 2983,RRID: AB_ 2105622 Rabbit polyclonal antiP-4EBP(S65) Cell signaling Technology Cat# 9451,RRID: AB_330947 Rabbit polyclonal anti4EBP (PHAS-1) Invitrogen Cat# 51-2900 Rabbit monoclonal anti-P-eIF2α (Ser51) Cell signaling Technology Cat#9721,RRID: AB_330951 rabbit monoclonal anti-eIF2α(D7D3) XP Cell signaling Technology Cat#9721,RRID: AB_10692650 Mouse monoclonal anti-eIF4E2 (Figs. 6, 7, and Additional file 1: Figure S1) Invitrogen Cat#MA5-25412 Mouse monoclonal anti-GAPDH (Fig. 5C and Additional file 1: Figure S1) Fitzgerald Cat# 10R-G109A, RRID: AB 1285808 Goat polyclonal antiVEGF-C R & D systems Cat# AF752-SP Rabbit polyclonal antiRibosomal protein L 7a (E109) Cell signaling Technology Cat# 2415,RRID:AB_ 2182059 Rabbit polyclonal anti-eIF4G Gift from Dr. Rhoads (LSUHSC, Shreveport) Mouse monoclonal anti-eIF4A Rabbit polyclonal anti-Actin Sigma Cat# A2066, RRID: AB_476693 Chemicals Dulbecco’s modified eagle medium (DMEM) Sigma-Aldrich D6429 Antibiotic–Antimycotic (100X) ThermoFisher Scientific 15240-062

Techniques: Binding Assay, Western Blot, Control

Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, eIF4E2, and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal ( A ) and hypoxic ( B ) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, eIF4E2, and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal ( A ) and hypoxic ( B ) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) , Cell signaling Technology , Cat# 6916, RRID: AB_10839268.

Techniques: Western Blot, Control

Nsp2 may affect composition of the cap-binding complexes. ( A ) Left, the representative images of western-blot analyses of proteins expressed in control and Nsp2-cells under normal and hypoxic conditions. Arrow indicates position of Strep-Nsp2. Right, ratio of densitometric signal of P-4EBP to total 4EBP in cells grown under normal or hypoxic conditions normalized to that in control cells grown under normal conditions, set as one (± SD, n = 3). ( B ) Left, the representative images of western blot analysis of proteins bound to m 7 GTP-Sepharose under normal and hypoxic conditions in control and Nsp2-cells. Arrow indicates position of Strep-Nsp2. Right, ratios of densitometric signals of eIF4G (upper graph) and eIF4E2 (lower graph) to eIF4E bound to m 7 GTP-Sepharose in experiments presented in lanes 1–4 (± SD, n = 3). Graph eIF4E/eIF4G: p = 0.014 for C–H versus C–N; p = 0.033 for Nsp2-H versus C–H. Graph eIF4E2/eIF4E: p = 0.03 for C–H versus C–N; p = 0.025 and 0.023 for Nsp2–H versus C–N and versus C–H, respectively

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Nsp2 may affect composition of the cap-binding complexes. ( A ) Left, the representative images of western-blot analyses of proteins expressed in control and Nsp2-cells under normal and hypoxic conditions. Arrow indicates position of Strep-Nsp2. Right, ratio of densitometric signal of P-4EBP to total 4EBP in cells grown under normal or hypoxic conditions normalized to that in control cells grown under normal conditions, set as one (± SD, n = 3). ( B ) Left, the representative images of western blot analysis of proteins bound to m 7 GTP-Sepharose under normal and hypoxic conditions in control and Nsp2-cells. Arrow indicates position of Strep-Nsp2. Right, ratios of densitometric signals of eIF4G (upper graph) and eIF4E2 (lower graph) to eIF4E bound to m 7 GTP-Sepharose in experiments presented in lanes 1–4 (± SD, n = 3). Graph eIF4E/eIF4G: p = 0.014 for C–H versus C–N; p = 0.033 for Nsp2-H versus C–H. Graph eIF4E2/eIF4E: p = 0.03 for C–H versus C–N; p = 0.025 and 0.023 for Nsp2–H versus C–N and versus C–H, respectively

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) , Cell signaling Technology , Cat# 6916, RRID: AB_10839268.

Techniques: Binding Assay, Western Blot, Control

Expression of Nsp2 and its binding to eIF4E2. A Representative image of western blot analysis of expression of Strep-Nsp2 in HEK293T cells stably transfected with either Lipofectamine-3000 or LTX. B Biotin-Agarose pull-down. Left, western blot analyses of eIF4E2 (upper panel) and Nsp2 (lower panel). Right, graph represents densitometric ratio of eIF4E2 to Nsp2. C m 7 GTP-Sepharose pull-down. Left, western blot analyses of Nsp2 (upper panel) and eIF4E2 (lower). Right, graph represents densitometric ratio of Nsp2 to eIF4E2. C—control; N—normoxia; H—hypoxia. The blots shown are representative of three completely independent experiments

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Expression of Nsp2 and its binding to eIF4E2. A Representative image of western blot analysis of expression of Strep-Nsp2 in HEK293T cells stably transfected with either Lipofectamine-3000 or LTX. B Biotin-Agarose pull-down. Left, western blot analyses of eIF4E2 (upper panel) and Nsp2 (lower panel). Right, graph represents densitometric ratio of eIF4E2 to Nsp2. C m 7 GTP-Sepharose pull-down. Left, western blot analyses of Nsp2 (upper panel) and eIF4E2 (lower). Right, graph represents densitometric ratio of Nsp2 to eIF4E2. C—control; N—normoxia; H—hypoxia. The blots shown are representative of three completely independent experiments

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) , Cell signaling Technology , Cat# 6916, RRID: AB_10839268.

Techniques: Expressing, Binding Assay, Western Blot, Stable Transfection, Transfection, Control

Nsp2 co-localizes with eIF4E2 and RER under normal and hypoxic conditions. A Strep-Nsp2 was probed using anti-strep antibody (green) and eIF4E2 probed using anti-eIF4E2 antibody (red), DAPI (blue) stained nuclei. Merge channel from max intensity projections of z-stacks under hypoxia shows increased co-localization (yellow). B Strep-Nsp2 (green) and calnexin (red) probed. Under hypoxia, granular staining in merged channel (yellow) shows increased co-localization from best focal plane. C Representative image of Ribosomal marker (RbS3) probed using anti-RbS3 antibody (red) and Strep-Nsp2 (green). Colocalization shown in merged channel. DAPI stained nuclei

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Nsp2 co-localizes with eIF4E2 and RER under normal and hypoxic conditions. A Strep-Nsp2 was probed using anti-strep antibody (green) and eIF4E2 probed using anti-eIF4E2 antibody (red), DAPI (blue) stained nuclei. Merge channel from max intensity projections of z-stacks under hypoxia shows increased co-localization (yellow). B Strep-Nsp2 (green) and calnexin (red) probed. Under hypoxia, granular staining in merged channel (yellow) shows increased co-localization from best focal plane. C Representative image of Ribosomal marker (RbS3) probed using anti-RbS3 antibody (red) and Strep-Nsp2 (green). Colocalization shown in merged channel. DAPI stained nuclei

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) , Cell signaling Technology , Cat# 6916, RRID: AB_10839268.

Techniques: Staining, Marker

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-4EHP (eIF4E2) , Cell signaling Technology , Cat# 6916, RRID: AB_10839268.

Techniques: Modification, Western Blot, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Luciferase, Software, Recombinant, Imaging, Microscopy

Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, eIF4E2, and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal ( A ) and hypoxic ( B ) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, eIF4E2, and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal ( A ) and hypoxic ( B ) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal

Article Snippet: Rabbit anti-eIF4E2 , Invitrogen , Cat# PA5-110849.

Techniques: Western Blot

Nsp2 may affect composition of the cap-binding complexes. ( A ) Left, the representative images of western-blot analyses of proteins expressed in control and Nsp2-cells under normal and hypoxic conditions. Arrow indicates position of Strep-Nsp2. Right, ratio of densitometric signal of P-4EBP to total 4EBP in cells grown under normal or hypoxic conditions normalized to that in control cells grown under normal conditions, set as one (± SD, n = 3). ( B ) Left, the representative images of western blot analysis of proteins bound to m 7 GTP-Sepharose under normal and hypoxic conditions in control and Nsp2-cells. Arrow indicates position of Strep-Nsp2. Right, ratios of densitometric signals of eIF4G (upper graph) and eIF4E2 (lower graph) to eIF4E bound to m 7 GTP-Sepharose in experiments presented in lanes 1–4 (± SD, n = 3). Graph eIF4E/eIF4G: p = 0.014 for C–H versus C–N; p = 0.033 for Nsp2-H versus C–H. Graph eIF4E2/eIF4E: p = 0.03 for C–H versus C–N; p = 0.025 and 0.023 for Nsp2–H versus C–N and versus C–H, respectively

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Nsp2 may affect composition of the cap-binding complexes. ( A ) Left, the representative images of western-blot analyses of proteins expressed in control and Nsp2-cells under normal and hypoxic conditions. Arrow indicates position of Strep-Nsp2. Right, ratio of densitometric signal of P-4EBP to total 4EBP in cells grown under normal or hypoxic conditions normalized to that in control cells grown under normal conditions, set as one (± SD, n = 3). ( B ) Left, the representative images of western blot analysis of proteins bound to m 7 GTP-Sepharose under normal and hypoxic conditions in control and Nsp2-cells. Arrow indicates position of Strep-Nsp2. Right, ratios of densitometric signals of eIF4G (upper graph) and eIF4E2 (lower graph) to eIF4E bound to m 7 GTP-Sepharose in experiments presented in lanes 1–4 (± SD, n = 3). Graph eIF4E/eIF4G: p = 0.014 for C–H versus C–N; p = 0.033 for Nsp2-H versus C–H. Graph eIF4E2/eIF4E: p = 0.03 for C–H versus C–N; p = 0.025 and 0.023 for Nsp2–H versus C–N and versus C–H, respectively

Article Snippet: Rabbit anti-eIF4E2 , Invitrogen , Cat# PA5-110849.

Techniques: Binding Assay, Western Blot

Expression of Nsp2 and its binding to eIF4E2. A Representative image of western blot analysis of expression of Strep-Nsp2 in HEK293T cells stably transfected with either Lipofectamine-3000 or LTX. B Biotin-Agarose pull-down. Left, western blot analyses of eIF4E2 (upper panel) and Nsp2 (lower panel). Right, graph represents densitometric ratio of eIF4E2 to Nsp2. C m 7 GTP-Sepharose pull-down. Left, western blot analyses of Nsp2 (upper panel) and eIF4E2 (lower). Right, graph represents densitometric ratio of Nsp2 to eIF4E2. C—control; N—normoxia; H—hypoxia. The blots shown are representative of three completely independent experiments

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Expression of Nsp2 and its binding to eIF4E2. A Representative image of western blot analysis of expression of Strep-Nsp2 in HEK293T cells stably transfected with either Lipofectamine-3000 or LTX. B Biotin-Agarose pull-down. Left, western blot analyses of eIF4E2 (upper panel) and Nsp2 (lower panel). Right, graph represents densitometric ratio of eIF4E2 to Nsp2. C m 7 GTP-Sepharose pull-down. Left, western blot analyses of Nsp2 (upper panel) and eIF4E2 (lower). Right, graph represents densitometric ratio of Nsp2 to eIF4E2. C—control; N—normoxia; H—hypoxia. The blots shown are representative of three completely independent experiments

Article Snippet: Rabbit anti-eIF4E2 , Invitrogen , Cat# PA5-110849.

Techniques: Expressing, Binding Assay, Western Blot, Stable Transfection, Transfection

Nsp2 co-localizes with eIF4E2 and RER under normal and hypoxic conditions. A Strep-Nsp2 was probed using anti-strep antibody (green) and eIF4E2 probed using anti-eIF4E2 antibody (red), DAPI (blue) stained nuclei. Merge channel from max intensity projections of z-stacks under hypoxia shows increased co-localization (yellow). B Strep-Nsp2 (green) and calnexin (red) probed. Under hypoxia, granular staining in merged channel (yellow) shows increased co-localization from best focal plane. C Representative image of Ribosomal marker (RbS3) probed using anti-RbS3 antibody (red) and Strep-Nsp2 (green). Colocalization shown in merged channel. DAPI stained nuclei

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet: Nsp2 co-localizes with eIF4E2 and RER under normal and hypoxic conditions. A Strep-Nsp2 was probed using anti-strep antibody (green) and eIF4E2 probed using anti-eIF4E2 antibody (red), DAPI (blue) stained nuclei. Merge channel from max intensity projections of z-stacks under hypoxia shows increased co-localization (yellow). B Strep-Nsp2 (green) and calnexin (red) probed. Under hypoxia, granular staining in merged channel (yellow) shows increased co-localization from best focal plane. C Representative image of Ribosomal marker (RbS3) probed using anti-RbS3 antibody (red) and Strep-Nsp2 (green). Colocalization shown in merged channel. DAPI stained nuclei

Article Snippet: Rabbit anti-eIF4E2 , Invitrogen , Cat# PA5-110849.

Techniques: Staining, Marker

Journal: Virology Journal

Article Title: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

doi: 10.1186/s12985-023-02021-2

Figure Lengend Snippet:

Article Snippet: Rabbit anti-eIF4E2 , Invitrogen , Cat# PA5-110849.

Techniques: Modification, Western Blot, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Luciferase, Software, Recombinant, Imaging, Microscopy